Rt-pcr as Effective Detection Method of Equine Arteritis Virus in the Semen of Naturally Infected Stallions
نویسندگان
چکیده
The aim of the study was to present the usefulness of one tube reverse transcriptase polymerase chain reaction assay (RT-PCR) in comparison to virus isolation test (VI) in the detection of equine arteritis virus. The group of 82 semen samples taken from different stallions was examined. Equine arteritis virus was isolated in RK-13 cell monolayers from the seminal plasma of 11 animals. The same results were obtained by one tube RT-PCR assay. The study showed that RT-PCR assay provides a sensitive, accurate, convenient and less time-consuming way for equine viral arteritis diagnosis in close tube system which minimises the risk of crosscontamination.
منابع مشابه
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